product information
Background

Histone H3-like centromeric protein HTR12 replaces formal H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for the assembly of kinetochore proteins, mitotic progression and chromosome segregation. Localized at centromeres in both m itotic and meiotic cells. Synonymes: CENH3

Immunogen KLH-conjugated synthetic peptide derived from N-terminal domain of Arabidopsis thaliana HTR12 AT1G01370 (Q8RVQ9)
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Store reconstituted antibodies at 4°C.

Shelf life is 6 months from a date of receipt.
Tested applications ELISA, IHC, WB
Related products

AS12 2226-200 | anti-HTR12 | histone H3-like centromeric protein (200 µl) antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 200 - 1:2000 (WB), 1: 50 - 1:500 (IHC)
Expected | apparent MW

17 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Crucihimalaya himalaica, Turritis glabra
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

This product can be sold containing ProClin if requested

Selected references

to be added when available, antibody released in August 2012.